Exploring Quantitative Biological Major, Trace, and Ultratrace Elements Composition and Qualitative Primary-Secondary Metabolites in Lamiaceae Medicinal Plants from Turkey.

Medicinal plants comprise a spectrum of constituents, encompassing both organic and inorganic elements. Elemental composition of 27 species of medicinal plants of Lamiaceae (including 17 endemic) family grown in Turkey was carried out by ICP-MS. The following elements were determined in analysed samples: Na, Mg, Al, K, Ca, Sc, Cr, Mn, Fe, Co, Zn, As, Rb, Sr, Cs, Ba, La, Ce, Sm, U, Se. Quantitative analysis of specific primary and secondary metabolites was carried out. Na and K are major constituents in plants. The concentrations of Na range from 332,495.590 g/kg (in sample 10SA) to 279,690.674 g/kg (in sample 4SA), while those of K vary from 67,492.456 g/kg (in sample 15SA) to 3347.612 g/kg (in sample 1A). Some metals such as Al, Cr, Mn, Fe, Co, Zn, As, Se, Rb, Sr, Cs, and Ba were also detected. Flavonoids, carbohydrates and tannins were present in all sample. Saponins were found in all samples except 1C and 2O. Coumarin were detected in samples 2N, 1 T, 1O, 1Z, 3SA, 1C, 4SA, 6SA, 8SA, 1 M, 11SA, 13SA, 2O, 14SA, 1H, and 16SI. Lipids were present in samples 6S, 9S, 1A, 10S, 1 M, 11SA, 12SA, 13SA, 14SA, and 16SI. Plants contain essential, rare earth, and trace elements at mg/kg concentrations, while major elements such as K and Na are present in high levels. Toxic element As (arsenic) was detected in all analyzed plants, but in most samples, its concentration was below the threshold set by World Health Organization.

Phytochemical Analysis and Evaluation of Genotoxic, Antigenotoxic Effects in Bacterial Models, and Hypoglycemic Activity of Epilobium angustifolium L. with Bioguided Isolation of Active Compounds.

This study examined the effects of methanol extract and its sub-extracts from Epilobium angustifolium on α-glucosidase and α-amylase activity. Secondary metabolites and amino acids were quantified using LC-MS/MS. Dichloromethane sub-extract displayed the highest activity and was chosen for further investigation. Despite the widespread use of E. angustifolium, genotoxicity studies were conducted to assess its safety. Dichloromethane significantly inhibited α-glucosidase (IC50 =17.340 µg/mL), making it approximately 293 times more effective than acarbose. Six known compounds, including gallic acid (1), a mixture of quercetin-3-O-α-galactoside (2a) and quercetin-3-O-α-glucoside (2b), quercetin-3-O-α-glucuronic acid (3), quercetin-3-O-α-rhamnoside (4), and kaempferol-3-O-α-rhamnoside (5) were identified. Quercetin-3-O-α-rhamnoside exhibited the highest inhibition of α-glucosidase (IC50 =1735±85 µM), making it 3.70 times more effective than acarbose. Dichloromethane also showed significant antigenotoxic activity against mutagenesis induced by NaN3, 9-AA, 4-NPD, and MNNG. Gallic acid was found in the highest abundance (13253.6931 ng/mL) in the methanolic extract. Furthermore, L-Aspartic acid was the most concentrated amino acid (363.5620 nmol/mL) in the methanolic extract.

Anatomical, Morphological, and Chemical Characterizations and Biological Activities of Gagea taurica Steven (Liliaceae): A New Record for the Turkish Flora.

Gagea genus, which is native to the Mediterranean and Black Sea regions, has attracted significant attention due to its biodiversity and potential health benefits. In this study, the biochemical composition and biological activities of methanol extracts from various parts of G. taurica were investigated, along with their anatomical and morphological characteristics. The best antimicrobial activity was found to be MeOH extracts of corm and leaf against several Candida strains with MIC=640 µg/mL. The highest level of phenolics together with significant results of antioxidant activities were observed in flowers extracts. The α-amylase inhibition assay results showed that the highest inhibition percentage was observed with acarbose (59 %), followed by leaf extract (43 %). Leaf exhibited the most effective inhibitory activity in AChE inhibition assay, whereas flower demonstrated the most significant inhibitory activity in BChE inhibition assay. Hesperidin was found as 1621.0001 ng/ml value in flower extract and 283.9339 ng/ml value leaf.

Phytochemical screening, biological evaluation, anatomical, and morphological investigation of Ferula tingitana L. (Apiaceae).

Ferula tingitana L. is a high perennial plant and its leaf is an alternate arrangement and yellow, and its flowers are unisexual like other Apiaceae. It has been used as a spice and for various medicinal purposes in the Mediterranean region. The paper reports antidiabetic, antimicrobial, anticholinesterase, antioxidant, and genotoxic activities of leaves, flowers, stems, and fruits methanol extracts of F. tingitana. Also, quantitative determination of some secondary metabolites was also analyzed by LC-MS/MS. Moreover, chemical composition of essential oils was analyzed. Consequently, anatomical, and morphological properties of plant were investigated. Germacrene D (23.6%), 1,3,5-trimethylbenzene (18.4%), and α-pinene (50.0%) were found as the main compounds in flower, leaf, and stem oils, respectively. The cortex in stem, pedicel, and fruit is characterized by angular collenchyma cells and a distinct cambium layer. 6 compounds (quinic acid, fumaric acid, keracyanin chloride, cyanidin-3-O-glucoside, chlorogenic acid, hesperidin) were observed in samples. Leaf extract showed anticholinesterase activity. Leaf and flower extracts showed the highest % inhibition value on ABTS·+ and DPPH•. Leaf extract has the strongest antioxidant effect because it is rich in total phenolic contents. All extracts of F. tingitana were found generally effective against C. albicans. Stem extract was found effective against E. coli and flower extract was found more effective against S. enterica and C. albicans. Bacterial genotoxicity results showed that extracts did not have genotoxic activity on tester strains S. typhimurium and E. coli WP2uvrA. Thus, it revealed that extracts were genotoxic-ally safe at applied concentrations up to 3 mg/plate.

The anatomical, morphological features, and biological activity of Scilla siberica subsp. armena (Grossh.) Mordak (Asparagaceae).

Scilla siberica subsp. armena is known as Siberian Squill which is naturally distributed in Lebanon-Syria, Transcaucasus, and Turkey. It is a perennial bulbous plant with a short vegetation period from March to May. In this study, the antimicrobial, antioxidant, α-amylase, and α-glucosidase inhibitory activities of corm, leaf, and flower methanolic extracts were examined. The anatomy and morphology of the plant organs were investigated by light and electron microscopes. The anatomy of S. siberica subsp. armena was investigated for the first time by electron microscopy in this study. The corm, leaf, and flower methanolic extracts were assessed against E. coli ATCC 8739, S. aureus ATCC 6538, B. subtilis ATCC 19,659, C. albicans ATCC 10,231, C. krusei ATCC 14,243, and C. tropicalis ATCC 750. Among the extracts, that obtained from the blue pollen showed the best antimicrobial activity against C. tropicalis ATCC 750 strain with a MIC value of 312.5 µg/mL. The highest phenolic content was determined in leaf extract with 53.59211 µg GAE/mg extract value. The extract showed the best anti-lipid peroxidation activity with 376.69 µg/mL value. Using DPPH· and ABTS·+ tests, it was determined that the flower and leaf extracts have the best activity (IC50 = 756.13 µg/mL and IC50 = 94.07 µg/mL, respectively). The flower extract exhibited α-glucosidase inhibitory activity with the IC50 value of 5239 µg/mL. Based on the presented results of the in vitro antimicrobial, antioxidant, and antidiabetic activities of the S. siberica subsp. armena, we suggest that natural compounds from S. siberica subsp. armena are of potential use for the improvement of an antidiabetic, antioxidant, and antimicrobial agent.

Screening of antimicrobial, antioxidant, antidiabetic activities, anatomical and morphological properties of Colchicum speciosum Steven (Colchicaceae).

Colchicum speciosum Steven species is a perennial stemless plant. C. speciosum is a flowering herb native to mountainous regions of northern Turkey, the Caucasus, and northern Iran. It has been known as "Vargit, Aci Çigdem, Güz Çigdemi". The present study reports the antimicrobial, antioxidant, α-amylase and α-glucosidase inhibitory activities of corm, leaf and flower methanol extracts, anatomical (light and electron microscopes) properties of root, corm, leaf, flowers and morphological characteristics of C. speciosum. Three different part of extracts C. speciosum were evaluated against E. coli ATCC 8739, S. aureus ATCC 6538, B. subtilis ATCC 19,659, C. albicans ATCC 10,231, C. krusei ATCC 14,243, and C. tropicalis ATCC 750. The most effective extract was found to be MeOH extract for corm and leaf against C. tropicalis ATCC 750 strain with MIC value 160 > µg/mL. It has been investigated first time anatomy of the tepal, ovary, anther, filament of C. speciosum. Leaf extract was the highest phenolic component (78.61842 µg GAE/ mg extract). As a result of DPPH• and ABTS•+ tests, it was determined that the leaf extract showed the best activity (IC50 = 6.568 µg/mL and IC50 = 3.243 µg/mL, respectively). Corm extract exhibited α-glucosidase inhibitory activity with an IC50 value of 21039 µg/mL. This is the first study of the in vitro antimicrobial, antioxidant, antidiabetic activities, detailed anatomical and morphological properties of C. speciosum. HiGHLiGHTS : • Antioxidant-antidiabetic-antimicrobial potential of Colchicum speciosum • Leaf extract had the highest phenolic component • The leaf got the highest DPPH• and ABTS•+ antioxidant potential • Corm extract exhibited α-glucosidase inhibitory activity • The most effective extract was found to be MeOH extract for corm and leaf against C. tropicalis • This is the first study of the in vitro antimicrobial, antioxidant, antidiabetic activities, detailed anatomical and morphological properties of C. speciosum.